Question of the day (14/12/16)

A bacterium that can live in alkaline pH is

a.      Escherechia coli

b.      Salmonella typhii

c.       Shigella flexneri

d.      Vibrio cholorae

Please comment ur answers

Question for the day (13/12/16)

Whooping cough is caused by

1.      Boedetella pertusis

2.      Streptococcus pneumonia

3.      Streptococcus agalactae

4.      Bordetella bronchiseptica

http://refreshbio.blogspot.in/

Question of the day II (12/12/16)

In bacterial replication the DNA polymerase I has

a.       3’-5’ Exonuclease activity

b.      5’-3’ Exonuclease activity

c.       Has 1 sub unit

d.      All the above

Please comment ur answers

A MCQ exam based on CSIR UGC (Life sciences)

A MCQ exam based on CSIR UGC (Life sciences) is scheduled on (13/12/16 7 AM till 14/12/16 7 AM). 

Ø  Only registered candidates will be allowed

Ø  Please comment ur mail Id for registration

Ø  Also register in refreshingbio@gmail.com

Today’s question (12/12/16)

The special present in amino acids arginine and tyrosine are

1.      Phenol and Guanidium

2.      Imidazole and guanidium

3.      Inidazole and guanidium

4.      Guanidium and phenol

Please comment ur answers…..

Question for the day (11/12/16)

1.       Oxidation of 1 NADH and 1 FADH₂ molecule yields ______________ ATP molecules??

A.3&2.    B.2&3     C.1&2     D. 3&3

Please comment ur answers

Please visit http://refreshbio.blogspot.in/

Todays question (10/12/16)

 Which one of the following is not an essential ingredient for PCR??

1.      Taq Polymerase 2. rNTP 3. MgCl₂  4. Primers

                           

                                       Please comment ur answers

                            

CSIR Online test

Dear members

A MCQ test (online test) is scheduled from 12 noon tomorrow (10/12/16) till 12 noon Sunday (11/12/16).

The test will be online

Only registered candidates will be allowed

Candidates can register at refreshingbio@gmail.com

http://refreshbio.blogspot.in

CSIR NET online classes

CSIR test series I

CSIR test series I (from 7 pm today till 7 am tomorrow)

Interested people can attend
here is the link
https://www.classmarker.com/online-test/start/…

Interested people can contact refreshingbio@gail.com to get password

Question for the day 231116

Question of the day 231116

Mail ur answers refreshingbio@gmail.com

A freshly isolated plasmid by alkaline lysis method is run in an 0.8% agarose electrophoresis.  The plasmid was found to contain two restriction sites for HindIII one for BamHI and three for SphI. Subsequently the plasmid was digested BamH1 and was run in the same gel (above discussed gel). The uncut plasmid corresponds to 1kB whereas the digested plasmid corresponds to 4kB. What is the size of the plasmid??

Answers for the questions 221116

1. Cancerous cells mainly depend for their ATP through 
a. Glycolysis b. Anaerobic glycolysis c. Citric acid cycle d. all the above

Answer- The main source of ATP production in cancer cells is glycolysis and to an extent anaerobic glycolysis. Glycolysis in enhanced and beneficial to cancer cells

Physiology Lecture series II

Online course 
Please register at refreshingbio@gmail.com 
Course fee 2000/- for entire session

Answers for the question 201116

                                                     CSIR-UGC section

1.      In many organisms other than vertebrates the main aim of glyoxalate cycle is conversion of 

a.       Carbohydrate to acetyl CoA     b. acetate to carbohydrate

b.      Carbohydrate to acetate            d. None of the above. 

Answers

IN organisms other than vertebrate the glyoxylate cycle serves as mechanism of converting acetate to carbohydrate. Hence the answer for the above question is b

                                      AIPMT section

1.      In light reaction of photosynthesis, the cells absorb light and conserve it as

a.       ATP and NADPH   b. NADH and ATP c.  NADH and NADPH d. None of the above

Answers

IN light reaction of photosynthesis the cells absorb light ad conserve it as ATP and NADPH

Technical workshop in basic molecular techniques

Dear members

We are offering technical workshop in basic techniques in Molecular biology. The following are the techniques

Day	Experiment
1	Ø  Introduction Ø  Media preparation, sterilization and inoculation
2	Ø  Plasmid isolation /genomic DNA isolation Ø  Agarose gel electrophoresis Ø  Restriction digestion
3	Ø  Preparation for Competent cell Ø  Vector insert purification Ø  Ligation
4.	Ø  Competent cell preparation and transformation Ø  Primer designing and validation
5.	Ø  PCR Ø  Colony selection

1. There will be lecture session in between experiments

2. There will also be session to assess their knowledge in their concepts

3. Interested People can discuss for further process email – refreshingbio@gmail.com

CSIR full test series I

Question of the day 201116

Questions for the day 201116

General instructions

1. The questions will be updated daily.

2. Please email the answers to the email ID- refreshingbio@gmail.com before 12 noon next day.

3. Get your address registered for getting your questions to your mail ID daily.

4. The answers, explanation for the questions and the details of the winners will be published next day.  

                                                 CSIR-UGC section

1.      In many organisms other than vertebrates the main aim of glyoxalate cycle is conversion of

a.       Carbohydrate to acetyl CoA     b. acetate to carbohydrate

b.      Carbohydrate to acetate            d. None of the above.

                                      AIPMT section

1.      In light reaction of photosynthesis , the cells absorb light and conserve it as

a.       ATP and NADPH   b. NADH and ATP c.  NADH and NADPH d. None of the above

Answers 191116

CSIR-UGC section

1.       An organism that is pathogenic in urinary tract and non pathogenic in stool 

a.       E. coli                                  b. Salmonella 

b.      c. Vibrio                              d. Mycobacterium

The solution for the above question is E. coli. E. coli is the microbe which is causes UTI in urinary tract whereas it is mere a commensal in digestive tract.                                                  

                                                   AIPMT section

2.     Which of the following belongs to innate immunity?

            a. T_h cells b. T_c cells c. Nasal mucosa d. C cells  

The solution for the above question is Nasal mucosa which forms a part of innate immunity. Nasal mucosa present in the nasal lining naturally prevents the entry of antigens. 

Questions 191116

General instructions

1. The questions will be updated daily.

2. Please email the answers to the email ID- refreshingbio@gmail.com before 12 noon next day.

3. Get your address registered for getting your questions to your mail ID daily.

4. The answers, explanation for the questions and the details of the winners will be published next day.  

2016-11-19

                                                    CSIR-UGC section

1.       An organism that is pathogenic in urinary tract and non pathogenic in stool

a.       E. coli                                  b. Salmonella 

b.      c. Vibrio                              d. Mycobacterium

                                                   AIPMT section

2.     Which of the following belongs to innate immunity

            a. T_h cells b. T_c cells c. Nasal mucosa d. C cells  

Answers for 181116

CSIR-UGC section

1.       A bacterium that is capable of surviving inside the macrophages is

a.       E. coli                                  b. Salmonella 

b.      c. Vibrio                              d. Mycobacterium

Solution

 The answer for the  above question id option both b and d.  Both Salmonella and Mycobaterium are capable of intramacrophage survival.

The Best solution under the section is provided by Ms.Anupam Jain of Faridabad.

                              AIPMT section

2.       Which is a Gam (+) organism

a.        Escherichia coli b. Streptococcus pneumonia c. Vibrio cholorae d. Pseudomonas aeriginosa

Solution- The answer for the above question is B. It is only Gram positive bacterium in the list. Rest of all are Gram negative.

The Best solution under the section is provided by Rohit Reddy Mullala of vijayawada.

Csir test series I

Dear Members

CSIR Test Series I covering subjects Biochemistry (carbohydrate, protein and lipid chemistry) and animal physiology (digestive circulatory, excretory, respiratory) is schedule on 20 11 16, Sunday 6.PM.

Please register at refreshingbio@gmail.com

Question of the day 181116

General instructions

1. The questions will be updated daily.

2. Please email the answers to the email ID- refreshingbio@gmail.com before 12 noon next day.

3. Get your address registered for getting your questions to your mail ID daily.

4. The answers, explanation for the questions and the details of the winners will be published next day.  

2016-11-18

                                                    CSIR-UGC section

1.       A bacterium that is capable of surviving inside the macrophages is

a.       E. coli                                  b. Salmonella

b.      c. Vibrio                              d. Mycobacterium

                              AIPMT section

2.       Which is a Gam (+) organism

a.       Escherichia coli b. Streptococcus pneumonia c. Vibrio cholorae d. Pseudomonas aeriginosa

Answers and winners 171116

Answers and winners 171116

CSIR section

Which are the enzymes are elevated in bone and prostate cancers??

a.       Alkaline phosphatase and Acid phosphatase

b.      Aspartate amino transferase and Acid phosphatase

c.       Gamma – glutamyl transferase and Alkaline phosphatase

d.      Bilirubin and acid phosphatase

Answer- The enzymes that are increased in circulation in bone and prostate cancer is alkaline phosphatise and acid phosphatase respectively (‘a’ option).

Solution

Intracellular enzymes are those which are tissue specific the level of which are increased when the particular tissue damage. Certain enzymes have iso forms where each iso form is specific in a particular tissue.

The correct answer in this section is provided by Ms. Anuradha of Trichy

AIPMT section

1.      Deficiency of Vitamin B12 causes 

a.       Hemolytic anaemia

b.      Megaloblastic anaemia

c.       Pernicious anaemia

d.      All the above

Answer- the answer for the above question is both option b and c.

Solution-

1.      Pernicious anaemia is a condition where are there no healthy RBCs.

2.      This results in poor oxygen supply to the body.

3.      It is caused by poor absorption of VitB₁₂ (less intrinsic factor).

4.      The other anaemia is pernicious anaemia which is also due to VitB12 deficiency.

5.      The nuclear maturation is not on par with the cytoplasmic maturation. (hence appear as enlarged cells).

The correct answer in this section is provided by Ms. Roopa of Chennai.