Thursday, 24 November 2016

Question for the day 231116

Question of the day 231116
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A freshly isolated plasmid by alkaline lysis method is run in an 0.8% agarose electrophoresis.  The plasmid was found to contain two restriction sites for HindIII one for BamHI and three for SphI. Subsequently the plasmid was digested BamH1 and was run in the same gel (above discussed gel). The uncut plasmid corresponds to 1kB whereas the digested plasmid corresponds to 4kB. What is the size of the plasmid??

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