Dear members
We are offering technical workshop in basic techniques
in Molecular biology. The following are the techniques
|
Day
|
Experiment
|
|
1
|
Ø
Introduction
Ø
Media
preparation, sterilization and inoculation
|
|
2
|
Ø
Plasmid
isolation /genomic DNA isolation
Ø
Agarose gel
electrophoresis
Ø
Restriction
digestion
|
|
3
|
Ø
Preparation for
Competent cell
Ø
Vector insert
purification
Ø
Ligation
|
|
4.
|
Ø
Competent cell
preparation and transformation
Ø
Primer designing
and validation
|
|
5.
|
Ø
PCR
Ø
Colony
selection
|
1. There
will be lecture session in between experiments
2. There
will also be session to assess their knowledge in their concepts
3.
Interested People can discuss for further process email – refreshingbio@gmail.com
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